Figure 4
Surface phenotype of lung dendritic cells (DCs) in postasthmatic mice. Lung cells were prepared from postasthmatic mice 21 days following LACK (Leishmania analog of the receptors of activated C kinase) challenge, enriched from CD11c+ cells using magnetic beads, and analyzed by fluorescence-activated cell sorting (FACS). Data show representative FACS profiles upon staining with antibodies to CD11c and CD11b (upper panels). The gates corresponding to autofluorescent (R1) and non-autofluorescent (R2) cells are indicated, as well as those corresponding to autofluorescent CD11b+ (R3), autofluorescent CD11b− (R4), non-autofluorescent CD11b+ (R5), and non-autofluorescent CD11b− (R6) cells. Data also show representative FACS profiles upon staining with the indicated antibody (empty histograms) or isotype control (gray histograms) after gating on the indicated populations (lower panels).
