Figure 3
Sublingual administration of CyaA-E5-OVA improves lung function and reduces bronchial inflammation as well as Th2 responses in the lungs and cervical lymph nodes (CLNs). (a) Airway hyperresponsiveness was determined in ovalbumin (OVA)-sensitized mice sublingually treated with either phosphate-buffer saline (PBS), soluble OVA (2 and 500 μg), CyaA-E5 (50 μg), or CyaA-E5-OVA (50 μg) by measuring Penh values in response to methacholine as described in the methods section. (b) Eosinophil counts in bronchoalveolar lavages (BALs). (c) Interleukin (IL)-5, IL-10, and IL-13 production by restimulated CLN or lung cells. Data are representative of two independent experiments (with eight mice per experimental group). Results are shown as mean±s.e.m; n=8 mice per group. Data were compared using the nonparametric test (Kruskal–Wallis). NS, not significant. *P<0.05; **P<0.01.
