Figure 7
Oral/lingual CD11b+ macrophage-like cells promote the development of suppressive CD4+ T cells upon stimulation with Pam3CSK4, R848, or transforming growth factor-β (TGF-β). The suppressive capacity of T cells primed by oral/lingual CD11b+CD11c−MHC-II+ macrophages purified from oral/lingual or intestinal tissues in the presence of Pam3CSK4, R848, or TGF-β was evaluated by fluorescence-activated cell sorting (FACS) on carboxyfluorescein succinimidyl ester (CFSE)-labeled third-party ovalbumin (OVA)-specific naive CD4+ T cells stimulated with the OVA323–339 peptide. Ratios between 1:1 and 1:4 suppressive/responder cells were tested. Data are representative of three independent experiments.
