Figure 4
From: Protective role of Toll-like receptor 4 in experimental gonococcal infection of female mice
Expression of surface TLR2 does not differ between BALB/c and TLR4-mutant mouse strains. BMDMs were derived from BALB/c and BALB/c-Lpsd-mice as described in the text. Cells were stained for surface expression of TLR2 either at rest or after 24 h treatment with the agents noted above. (a) Shown above are FACS histograms for demonstrating staining using either the specific anti-TLR2 mAb (black line) or a matched isotype control (gray shading). The vertical axis represents the relative cell number, whereas the horizontal axis represents the intensity of fluorescence in the FL2 channel. The percentage of cells that are either negative or positive for TLR2 is shown for each histogram. (b) The bottom graph shows the median fluorescence as calculated from the histograms for each condition. Treatments were as follows: unstim, unstimulated cells; TNF-α, 40 ng ml−1; LPS, 100 ng ml−1; H8/Lip, 1 μg ml−1; GC lys (N. gonorrhoeae FA1090 crude lysates) MOI 10:1. These histograms are representative of two independent experiments. BMDM, bone marrow-derived macrophage; FACS, fluorescence-activated cell sorting; MOI, multiplicity of infection; TLR2, Toll-like receptor-2; TNF-α, tumor necrosis factor-α.
