Figure 5
From: Protective role of Toll-like receptor 4 in experimental gonococcal infection of female mice
Cells derived from TLR4-mutant mice are hyporesponsive to stimulation with N. gonorrhoeae. (a) BMDMs or (b) splenic mononuclear cells were derived from BALB/c and BALB/c-Lpsd mice as described in the text, and treated with the indicated agents. Supernatant was collected at 24 h for panel a and at the indicated time points for panel b, and assayed for TNF-α or IL-6 by ELISA. Pam2Cys, lipopeptide Pam2-Cys-Ser-Lys4; GC lys, N. gonorrhoeae FA1090 crude lysates. Significance was calculated using an unpaired t-test, with P-values as follows: **P<0.01; ***P<0.001; NS, not significant. These data are representative of two independent experiments. BMDM, bone marrow-derived macrophage; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; TLR4, Toll-like receptor-4; TNF-α, tumor necrosis factor-α.
