Figure 4
From: Altered distribution of mucosal NK cells during HIV infection
Flow cytometric confirmation of NKp46 antibody specificity for NK cells in the peripheral blood. To confirm that the two NKp46 antibodies specifically stained NK cells, the specificity of the antibodies was verified by flow cytometry using a panel of NK cell-specific antibodies. The overall frequency of CD3−CD16+/−CD56+/− NK cells stained with the traditional BD NKp46, the GAH NKp46, and the MAH NKp46 were similar. (a) Additional NK cell markers, including NKp30, NKG2A, KIR, NKG2D, were expressed at equivalent levels on blood NK cells labeled with the three different NKp46 antibodies. (b) Furthermore, lymphocytes isolated from the gut tissue were stained with the traditional BD NKp46, the GAH NKp46, and the MAH NKp46 to confirm that these antibodies label CD45posCD3neg NK cells in mucosal samples. GAH, goat anti-human; MAH, mouse anti-human; NK, natural killer.
