Figure 1
From: M cell-depletion blocks oral prion disease pathogenesis
Receptor activator of NF-κB ligand (RANKL) neutralization depletes glycoprotein 2 (GP2+) microfold cells (M cells) within the follicle-associated epithelium (FAE) of Peyer's patches. Whole-mount immunohistochemical analysis of the effect of anti-RANKL monoclonal antibody (mAb) treatment on the presence of GP2+ M cells within the FAE of Peyer's patches. (a) Tissues were stained with GP2-specific mAb (green), the lectin UEA-1 (red), and phalloidin (blue) to detect F-actin. The positions of the X–Z and Y–Z projections of the FAE are indicated by the solid line in the X–Y image. Arrows indicate GP2+ M cells with characteristic basolateral pockets. Arrowheads indicate GP2−UEA-1+ goblet cells. The boxed areas in each of the main panels are shown on the right-hand side at higher magnification with their X–Z and Y–Z projections. Scale bars: main panels, 100 μm; boxed areas, 25 μm. (b) The number of M cells in the FAE was significantly reduced after anti-RANKL mAb treatment (P=0.0079). Data are representative of 3–5 Peyer's patches from each of four control and four anti-RANKL mAb-treated mice.
