Figure 2
From: M cell-depletion blocks oral prion disease pathogenesis
Receptor activator of NF-κB ligand (RANKL) neutralization does not affect the status of follicular dendritic cells (FDC) or mononuclear phagocytes within the gut-associated lymphoid tissue (GALT) and spleen. (a) Immunohistochemical (IHC) analysis of the CD21/CD35-expressing FDC networks (red) within B-cell follicles (B220+ cells, green) in the GALT and spleens of anti-RANKL monoclonal antibody (mAb)-treated and control mice suggested no observable effect of treatment on FDC status. (b) No observable effect of RANKL neutralization on cellular prion protein (PrPC) expression (green) by FDC (CD35+ cells; red) was detected by IHC. (c) No observable effect of RANKL neutralization on the status of F4/80+ macrophages and CD11c+ cells in the intestine, GALT, and spleen. Dotted line (upper right panels) indicates luminal surface of follicle-associated epithelium. Boxed areas are displayed at higher power in underlying panels. Data are representative of tissues from four mice per group. Scale bars=50 μm unless stated otherwise. MLNs, mesenteric lymph nodes.
