Figure 3
From: Functional diversity of human vaginal APC subsets in directing T-cell responses
Functional specialties of the vaginal antigen-presenting cell (APC) subsets in directing CD8+ T-cell responses. 5,6-Carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled allogeneic naïve total T cells were cocultured for 7 days with the vaginal APC subsets or dendritic cells (DCs) generated in the presence of interferon (IFN)α (IFNDCs). (a) CD8+ T-cell proliferation was assessed by measuring CFSE dilution. Data are mean±s.d. of three independent experiments with duplicates. (b–e) After 7 days, T cells were stimulated with phorbol 12-myristate 13-acetate (PMA)/ionomycin in the presence of brefeldin A, and then stained for intracellular IFNγ, tumor necrosis factor-α (TNFα), and interleukin (IL)-5 expression. (b) Representative data of six independent experiments. (c) Summary of the data from six (IFNγ+ and IL-5+) and four (TNFα+) independent experiments using cells from different donors. (d) IFNγ+ and IL-5+ CD8+ T cells (N=6) or (e) IFNγ+ and TNFα+ CD8+ T cells induced with different APC subsets (N=3). *P<0.05 by analysis of variance (ANOVA) test. LC, Langerhans cells; LP, lamina propria; Mφ, macrophages.
