Figure 3
From: Migratory properties of pulmonary dendritic cells are determined by their developmental lineage
Ccr7 expression in CD11bhi dendritic cells (DCs) is restricted to Ly-6CloCD14med/lo DCs. (a–c) Co-production of CCR7-GFP (CCR7-green fluorescent protein) with I-A (a), Ly-6C (b), or CD14 (c) in the indicated pulmonary DC subsets 24 h after instillation of lipopolysaccharide (LPS) or polyinosinic-polycytidylic acid (poly (I:C)). (n=3 mice/group). A representative result of two independent experiments is shown. (d) Gating strategy for purifying CD14hiCD11bhi, CD14med/loCD11bhi, and CD103+ DCs from the lung (left) and expression of CCR7-GFP before and after ex vivo culture of the cells in LPS-containing media (right). Levels of CD86 are also shown. (e) Compiled data (n=2/group) of CCR7-GFP expression in the indicated DC subsets following LPS stimulation ex vivo. N/A, not applicable.
