Figure 4
From: Migratory properties of pulmonary dendritic cells are determined by their developmental lineage
Analysis of three distinct subpopulations of CD11bhi dendritic cells (DCs) in the lung. (a) Cytograms showing CD14 and Ly-6C staining of DCs within a CD11bhiCD11c+I-A+ gate at the indicated times following ovalbumin/lipopolysaccharide (OVA/LPS) instillation and (b) absolute number of cells in each subpopulation. (c) Cytograms showing CD103 and CD11b staining of total lung DCs in wild-type and Ccr2−/− mice (left). Also shown are numbers of total, CD11bhi, and CD103+ lung DCs under steady-state conditions and 16 h after OVA/LPS instillation (right) (n=3). (d) Cytograms showing CD14 and Ly-6C staining of CD11bhi lung DCs in wild-type and Ccr2−/− mice (left). Numbers of Ly-6Chi, CD14hi, and CD14med/lo DCs under steady-state conditions and 16 h after OVA/LPS instillation are shown (right) (n=3). (e) Ccr7 mRNA levels in the indicated lung DC subsets of LPS-treated mice (n=2 to 7). (f) Number of PKH+ migratory CD11bhi and CD103+ DCs in the mediastinal lymph nodes (LNs) of wild-type and Ccr2−/− mice 24 h after LPS instillation (n=3). A representative result of two independent experiments is shown. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NS, not significant.
