Figure 5
From: Migratory properties of pulmonary dendritic cells are determined by their developmental lineage
Inflammatory dendritic cells (DCs) in the lung do not migrate to mediastinal lymph nodes (mLNs). (a) Timeline for instillation of lipopolysaccharide (LPS) and PKH. (b) Ly-6C and CD14 display on PKH+ CD11bhi DCs from the lung (top) and mLNs (middle). Representative cytograms for individual mice are shown, as well as compiled results for five mice. Also shown is the ratio of the PKH+ cells in mLNs and the lung (bottom). (c) Expression of Ccr7-gfp and staining for Ly-6C on CD11bhi mLN DCs of Ccr7gfp/+ mice (n=2). (d) Total and Ly-6Chi CD11bhi inflammatory DCs in mLNs following instillation of either low- (0.1 μg) or high (10 μg)-dose LPS. (e) Ly-6C and PKH staining of CD11bhi DCs in mLNs after LPS instillation. Representative cytograms and compiled results (n=3) are shown. (f) Accumulation of inflammatory monocytes in mLNs 2 h after intravenous injection of 106 CFSE (carboxyfluorescein diacetate succinimidyl ester)-labeled Ly-6ChiCD11bhi bone marrow cells into recipient mice. Animals were instilled with the indicated amount of LPS 24 h before monocyte transfer (n=2). o.p., oropharyngeal; OVA, ovalbumin.
