Figure 6
From: Migratory properties of pulmonary dendritic cells are determined by their developmental lineage
Ly-6Chi and CD14hi cells in CD11bhi dendritic cells (DCs) are monocyte-derived. (a) Analysis of major lung DC subsets in wild-type and Flt3L–/– mice. Cytograms showing CD103 and CD11b staining of total CD11c+I-A+ lung DCs (left) and histograms of total, CD11bhi, and CD103+ lung DCs under steady-state conditions and 16 h after ovalbumin/lipopolysaccharide (OVA/LPS) instillation (right) (n=3). (b) Cytograms showing CD14 and Ly-6C staining of CD11bhi lung DCs (left) and histograms of Ly-6Chi, CD14hi and CD14med/lo DCs under steady state conditions and 16 h after OVA/LPS instillation (right) (n=3). (c) Cytograms show gating of donor-derived DCs (CD45.1+CD11bhiCD11c+I-A+) (top), and display of CD14 and Ly-6C on those DCs (bottom), following adoptive transfer of CD45.1 Ly-6Chi monocytes (CD3−CD11b+CD11c−CD19−CD115+I-A−Ly-6G− TER119−7-AAD−). Recipient CD45.2 mice were instilled with OVA/LPS 2 hrs prior to the monocyte transfer. (d) Histograms showing the number of monocyte-derived DCs corresponding to the indicated populations (n=3). A representative result of three independent experiments is shown.
