Figure 7
From: Migratory properties of pulmonary dendritic cells are determined by their developmental lineage
FLT3L/FLT3L-independent, monocyte-derived CD11bhi dendritic cells (DCs) are non-migratory. (a) Ccr7 mRNA levels in lung DC subsets of wild-type and Flt3L−/− mice as measured by qPCR (n=2 to 9). (b, c) PKH+ migratory CD11bhi DCs in mediastinal lymph nodes (mLNs) 24 h after lipopolysaccharide (LPS) instillation into airways of wild-type and Flt3L–/– mice (n=3). A representative result of two independent experiments is shown. (d) Migration of wild-type (CD45.1) bone marrow-derived DCs (BMDCs) to mLNs in wild-type and Flt3L–/– mice (both CD45.2) following airway instillation of the donor DCs followed by ovalbumin/LPS 4 h later. Analysis of mLNs from recipient mice was performed 20 h later by flow cytometry. (e-g) Migration assay of Flt3−/− DCs from lung to mLNs. (e) Mixed BM chimeric mice were generated by transfer of a mixture of wild-type (CD45.2) and Flt3−/− (CD45.1) BM cells into irradiated wild-type (CD45.2) mice. Lung and mLN DCs in the recipients were analyzed 16 h after PKH instillation to airway. Flow plots of cells displaying CD45.1 or CD45.2 in PKH-gated CD11bhi and CD103+ DCs are shown. Percentage (f) and absolute number (g) of PKH+ wild-type and Flt3−/− DCs in lung and mLNs of the chimeric mice. A representative result of two independent experiments is shown. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; N/A, not applicable; NS, not significant.
