Figure 1
IL-4 drives intestinal mastocytosis. (a) Chloroacetate esterase staining for mast cells (red, examples highlighted by red arrows) in jejunal sections in wild-type, interleukin (IL)-4-treated, or Il4rαF709 mice. (b) Representative flow cytometry diagrams and summary data of small intestinal mast cells (c-Kit+IgE+lin−CD45+) from wild-type and Il4rαF709 mice, gating on live lin−CD45+ cells (n=5). (c) IL-4 treatment dramatically expands intestinal mast cells in wild-type mice. IL-4 was injected as a cytokine-antibody complex with 2 μg IL-4 and 10 μg anti-IL-4 intraperitoneally on days −1, −4, and −7. Statistical analysis by unpaired t test, two-tailed. Data are representative of at least three experiments. Mast cell frequencies are expressed as the fraction out of lin−CD45+ cells. Ig, immunoglobulin.
