Figure 4
Phenotype of penile macrophages and T cells. Representative dot plots derived from urethral single-cell suspensions showing the presence of CD163+ macrophages (a left, R1 gate) or CD3+ T cells (b left, R2 gate) vs. isotype controls. Representative histograms of R1-gated CD163+ macrophages (a right, n=3) derived from all penile regions show cells staining positive for CD4 or CCR5 vs. isotype control. Representative plots of R2-gated urethral CD3+ T cells, also expressing either CD8 or CD4 (b right, n=2), show cells staining positive for either CD45RO or CCR7. Numbers represent the mean±s.e.m. proportions of positive cells. The graph in b shows mean±s.e.m. proportions of R2-gated CD3+ T cells derived from all penile regions expressing CCR5 (hatched bars), CD8 (black bars) or CD4 (light gray bars) derived from n=5 different penile tissues, as well as the memory T-cell marker CD45RO on CD8+/CD4+ T cells (white and dark gray bars, respectively) from n=2 additional penile tissues.
