Figure 4
C5aR−/− dendritic cells (DCs) show a regular maturation profile but produce less Th17-promoting cytokines in vitro. (a) Expression profile of major histocompatibility complex II (MHC-II) and costimulatory molecules on wild-type (wt) and C5aR−/− bone marrow (BM)-derived DCs following stimulation with fluorescein isothiocyanate-ovalbumin (FITC-OVA) for 24 h. Gray histograms depict the isotype control. The gray and the black lines represent the expression before and after OVA stimulation, respectively. Histograms are representative of three experiments. (b) Frequency of CD11b+, MHC-II+, CD80+, CD86+, or CD40+ DCs in BM cultures from wt and C5aR−/− mice. (c) Relative expression of CD11b, MHC-II, CD80, CD86, or CD40 as compared with isotype control staining (ΔMFI (mean fluorescent intensity)). (d) Cytokine profiles from BM-derived DCs after OVA stimulation for 24 h. Values shown are the mean±s.e.m.; n=3–6 per group, d.l. indicates that the mean is below detection limit, *P<0.05, **P<0.01, ***P<0.001. IL, interleukin; TGF, transforming growth factor.
