Figure 5
C5aR−/− dendritic cell (DC)-activated CD4+ T cells produce less interleukin (IL)-17A and interferon (IFN)-γ and suffer from accelerated cell death. (a) Spleen derived carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled CD4+ T cells from OVA-TCR (ovalbumin–T-cell receptor) transgenic DO11.10/Rag2−/− cells were co-cultured for 4 days in the presence of bone marrow (BM)-derived DCs with or without OVA (1 μM). CFSE expression in CD4+ cells was evaluated by flow cytometry. Histograms are representative of four independent experiments. (b) Cytokine profiles from co-cultures of BM-derived DCs with OVA-TCR transgenic DO11.10/Rag2−/− CD4+ T cells after OVA stimulation for 4 days. (c) CD4+ T-cell survival as assessed by 4′,6-diamidino-2-phenylindole (DAPI) staining after 7 days of co-culture. Histograms are representative of five independent experiments. (d) Levels of IL-2 in supernatants collected after 7 days of co-culture. (e) Expression profile of pro- and anti-apoptotic molecules evaluated by real-time reverse transcriptase–PCR. Values shown are the mean±s.e.m.; n=4, d.l. indicates that the mean is below detection limit, *P<0.05, **P<0.01, ***P<0.001. WT, wild type.
