Figure 7

Bone marrow (BM) cultures from C5aR^−/− mice show an increased frequency of CD11b^hiCD11c^intGr-1⁺F4/80⁺ suppressor cells (SCs). (a) (first panel on the left) BM cells were first gated on CD11c⁺CD11b⁺ cDCs. Within this population, SSC^hiGr-1⁺ cells were identified (second panel on the left), red cells in the first left panel represent the SSC^hiGr-1⁺ cells backgated to the CD11c⁺CD11b⁺ cell population; (third left panel) histogram showing the F4/80 expression within the CD11b⁺CD11c⁺Gr-1⁺ cell population; (right panel) frequency of CD11b^hiCD11c^intGr-1⁺F4/80⁺ cells in BM cultures from wild-type (wt) and C5aR^−/− mice. Data are representative of three independent experiments. (b) Morphology of purified C5aR^−/− SCs (May-Grünwald–Giemsa staining, original magnification × 400). (c) Relative expression of arginase 1 and nitric oxide synthase 2 (NOS2) mRNA±50 ng ml⁻¹ interleukin (IL)-4 from SCs as evaluated by real-time reverse transcriptase–PCR. Values shown are the mean±s.e.m.; n⩾3 per group, *P<0.05, **P<0.01, ***P<0.001.

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