Figure 3

Interleukin (IL)-33-producing dendritic cells in murine experimental allergic conjunctivitis (EAC). The EAC model was induced in BALB/c mice sensitized and topically challenged by SRW pollen (EAC), with phosphate-buffered saline (PBS)-treated mice (PBS) as controls. Frozen sections of eyeballs ((a) showing cornea and conjunctiva) and (b) cervical lymph nodes (CLNs) were used for double immunofluorescent staining with CD11c (Red) and IL-33 (Green) using DAPI (4′,6-diamidino-2-phenylindole) counterstaining (Blue). Arrows indicate positive staining signals. Each bar in the diagrams represents mean±s.d. of three independent experiments. *P<0.05; **P<0.01.