Figure 4
Interleukin (IL)-33 is induced in dendritic cells (DCs) via Toll-like receptor (TLR)/nuclear factor (NF)-κB signaling pathways. (a) The mRNA expression levels of MyD88 (myeloid differentiation primary-response protein 88), NF-κB1, NF-κB2, RelA, and IL-33 were evaluated by quantitative real-time PCR in DCs from BALB/c mice treated with flagellin (10 μg ml−1) with or without TLR5 antibody or quinazoline (NF-κB-I) 1 h preincubation for 8 h. (b) Representative images showing NF-κB p65 nuclear translocation and IL-33 production by immunofluorescent staining (Green) with propidium iodide counterstaining (Red) in DCs treated as described in a for 24 h. *P<0.05; **P<0.01, compared with control; ^P<0.05; ^^P<0.01, compared with flagellin. The color reproduction of this figure is available on Mucosal Immunology journal online.
