Figure 6
Interleukin (IL)-33 activates dendritic cells (DCs) in vitro. (a) The expression of cytokines and chemokines was evaluated by quantitative real-time PCR in DCs from BALB/c mice exposed to IL-33 (1 ng ml−1) for 4 h without or with 1 h previous incubation of ST2 antibody (Ab) or soluble ST2 protein. (b) Flow cytometry showed the enhanced expression of CD40, CD80, and OX40L of DCs exposed to IL-33 for 24 h. Each bar in the diagrams represents mean±s.d. of three independent experiments. *P<0.05; **P<0.01, ***P<0.001, compared with control; ^P<0.05, compared with IL-33. APC, allophycocyanin; CCL, C-C motif chemokine ligand; FITC, fluorescein isothiocyanate; MFI, mean fluorescent intensity; PE, phycoerythrin; SSC-A, side scatter area; TNF, tumor necrosis factor.
