Figure 5
Interferon (IFN)-α protein is detectable in lysates from the duodenal mucosa of most celiac disease (CD) patients by western blot at 18–20 kDa. In all, 1–3 biopsy specimens were lysed and cleared by centrifugation. Lysates containing 200 μg total protein were loaded onto each lane of a 15% sodium dodecyl sulfate gel. IFN-α was detected with mouse monoclonal antibodies after transferring the proteins onto a nitrocellulose membrane. Molecular weight markers indicated on the left. Lanes 1–2: positive control, 100 and 10 pg recombinant IFN-α2a (Roceron; Roc.); lanes 3–5: lysates from three untreated celiac disease (UCD) patients; lanes 6–7: lysates from two control patients; and lane 8: positive control containing 1 ng of a mix of various IFN-α subtypes (from Mabtech; Mabt.). Of note, there is an unspecific band at about 60 kDa in all lysates.
