Figure 2
Lack of CCR7 causes an increase in lamina propria (LP) but not in intraepithelial T lymphocytes. (a) Paraffin-embedded sections of the large intestine of 8–12-weeks-old wild type (Wt) or CCR7−/− mice (n=6–7 per group) were immunohistochemically stained for B (B220 in blue) and T (CD3 in red) cells. Representative stainings are shown. Bar=100 μm. (b) Mean number of LP CD3+ T lymphocytes/10 high-power fields (hpf) and of caspase3+ cells/10 hpf (c) in colon sections of Wt (n=6; black bars) and CCR7−/− (n=7; open bars). (d) Colonic tissues of both groups were stained with periodic acid-Schiff (PAS) reagent and PAS staining was quantified based on two sections of each Wt (n=6; black bars) and CCR7−/− mouse (n=7; open bars). (e) Mean number of intraepithelial CD3+ T lymphocytes/100 epithelial cells (EC) and of caspase3+ cells/100 EC (f) in colon sections of Wt (n=6; black bars) and CCR7−/− (n=7; open bars). Bars represent means±s.d. *P<0.05; Student's t test.
