Figure 5
CCR7−/− mice exhibit downregulated colonic chloride secretion and decreased cystic fibrosis transmembrane conductance regulator (CFTR) mRNA levels compared with Wt mice. (a) Colons of 10–12-week-old Wt (black bars; n=8) and CCR7−/− (open bars; n=8) mice were mounted in Ussing chambers and electrogenic chloride secretion was measured as ΔIsc after stimulation with theophylline (10 mM) and prostaglandin E2 (2.8 nM). Bars represent means±s.e.m. *P<0.05; Student's t-test. (b) Quantification of mRNA expression of CFTR by real-time quantitative reverse transcription PCR in distal colonic samples of 4 week- and 8-week-old CCR7−/− (open circles) compared with Wt (black squares) mice. Data are normalized to villin expression and expressed as means. *P<0.05; Mann–Whitney test.
