Figure 6
CCR7−/− mice exhibited decreased α-, β-, and γ-epithelial sodium channel (ENaC) subunit mRNA levels and reduced electrogenic sodium transport. (a) Colons of 10–12-week-old CCR7−/− (open squares and open bars; n=10) and Wt (black squares and black bars; n=8) mice were mounted in Ussing chambers. Electrogenic sodium transport was measured as monovalent cation flux (μmol h−1 cm−2) after induction of ENaC by aldosterone and consecutive inhibition by amiloride. The course of monovalent cation flux (JNa) of a representative Ussing experiment after addition of aldosterone is shown to the left. Averaged monovalent cation fluxes are shown in the bar diagram to the right. Bars represent means±s.e.m. *P<0.05; unpaired Student's t test. (b) Quantification of mRNA expression of the ENaC ion channel subunits ENaC-α, -β, and -γ by real-time quantitative reverse transcription PCR in distal colonic samples of 8-week-old CCR7−/− (n=5; open circles) compared with Wt (n=5; black squares) mice. Data are normalized to villin expression and expressed as means. *P<0.05 and **P<0.01; unpaired Student's t test.
