Figure 2
Developmental profile of lung inflammation in C57BL/6N Scnn1b-transgenic (Scnn1b-Tg+) mice. (a–d) Longitudinal bronchoalveolar lavage (BAL) cell counts in Scnn1b-Tg+ mice (▪) and wild-type (WT) littermates (□). n=6 and 8 at 5 days (5d); n=7 and 12 at 10 days (10d); n=12 and 13 at 4 weeks (4wk); n=10 and 8 at 8 weeks (8wk); n=4 and 4 at 6 months (6mo); n=4 and 6 at 12 months (12mo), for WT and Scnn1b-Tg+, respectively. (e, f) Longitudinal keratinocyte-derived cytokine (KC) and tumor necrosis factor α (TNFα) levels in BAL fluid (BALF). n=4 WT and 6 Scnn1b-Tg+ mice. Student's t test **P<0.005, *P<0.05 vs. WT littermates. (g, h) Macrophage size distribution in 5-day and 4-week-old C57BL/6N Scnn1b-Tg+ mice and WT littermates. n=6 WT and 8 Scnn1b-Tg+ mice at postnatal day (PND) 5; 10 WT and 13 Scnn1b-Tg+ mice at PND 28. Boxed regions highlight the percentage of total macrophages larger than the 90th percentile in WT mice. Student's t test **P<0.005, *P<0.05 vs. WT littermates.
