Figure 4
Genetic deletion of myeloid differentiation factor 88 (MyD88) reduces lung neutrophilia in Scnn1b-transgenic (Scnn1b-Tg+) mice, but does not blunt macrophage activation. (a–d) Differential bronchoalveolar lavage cell counts. n=8, 9, 8, and 8 at 5 days (5d), n=14, 9, 12, and 12 at 10 days (10d), n=5, 5, 5, and 9 at 4 weeks (4wk), and n=3, 6, 7, and 8 at 8 weeks (8wk) for MyD88+/−;Scnn1b-Tg−, MyD88−/−;Scnn1b-Tg−, MyD88+/−;Scnn1b-Tg+, and MyD88−/−;Scnn1b-Tg+ mice, respectively. Analysis of variance (ANOVA) **P<0.005, *P<0.05 vs. MyD88+/−;Scnn1b-Tg− mice. #P<0.05 vs. MyD88+/−;Scnn1b-Tg+ mice. (e) Macrophage size distribution in 4-week-old mice from the MyD88−/−×Scnn1b-Tg+ cross. Boxed regions highlight the percentage of total macrophages larger than the 90th percentile in MyD88+/−;Scnn1b-Tg− mice. n=5 MyD88+/−;Scnn1b-Tg−, 4 MyD88−/−;Scnn1b-Tg−, 5 MyD88+/−;Scnn1b-Tg+, and 8 MyD88−/−;Scnn1b-Tg+. ANOVA **P<0.005, *P<0.05 vs. MyD88+/−;Scnn1b-Tg− littermates. BALF, bronchoalveolar lavage fluid.
