Figure 4
IL-6 deficiency does not interfere with neutrophil release or recruitment to the lung. (a) Neutrophil numbers in total bone marrow from non-infected WT and IL-6 KO mice (n=3). (b) Frequency of neutrophils (cell number per ml) in peripheral blood of non-infected WT and IL-6 KO mice (n=3). (c) Levels of KC and MIP-2, in BALF from WT and IL-6 KO mice (n=5) 3 days p.i. with the H1N1 PR8 virus. (d) Levels of G-CSF in BALF from WT and IL-6 KO mice (n=5) 3 days p.i. with the H1N1 PR8 virus. (e) Frequency of neutrophils (cell number per ml) in peripheral blood of WT and IL-6 KO mice (n=4) 3 days p.i. with the H1N1 PR8 virus. (f) Survival curve of IL-6 KO mice (n=4) administered i.p. with G-CSF (5 μg per mouse) or PBS control a day before infection and at day 3 p.i. (g) Neutrophils in BAL from WT and IL-6 KO mice (n=3) 24λh after administration of nebulized LPS. *P<0.05, NS denotes “not significant”. Statistical significance was determined by Student's t-test (panels a–e and g), or log-rank test (panel f). Results are representative of at least two independent experiments. BALF, bronchoalveolar lavage fluid; G-CSF, granulocyte colony-stimulating factor; IL, interleukin; i.p., intraperitoneal; KO, knockout; LPS, lipopolysaccharide; PBS, phosphate-buffered saline; p.i., post infection; WT, wild type.
