Figure 6
IL-6 prevents downregulation of anti-apoptotic molecules by influenza virus in neutrophils. (a) Neutrophils from WT mice were cultured in the presence or absence of the PR8 H1N1 virus at 1:10 neutrophil:virus (EIU) ratio with or without IL-6 (20 ng ml−1). After 18 h, mitochondrial ROS was examined by staining with MitoSox-Red dye and flow cytometry analysis. Histogram profiles for neutrophils cultured in medium (thin line), with H1N1 virus (thick line) or H1N1 virus and IL-6 (filled) are shown. (b) Western blot analysis for Mcl-1 and Bcl-XL in neutrophils from WT mice treated as described in panel a. Actin was used as loading control. IL, interleukin; ROS, reactive oxygen species; WT, wild type.
