Figure 2
From: Lung B cells promote early pathogen dissemination and hasten death from inhalation anthrax
Rapid transport of Bacillus anthracis (BA) spores to the lung-draining lymph nodes (LDLNs) is independent of CCR7 (chemokine (C-C motif) receptor 7). (a) C57BL/6 (n=4) or B6.CCR7−/− (n=4) mice were infected intratracheally (i.t.) with 3 × 108 DyLight 649-labeled BA spores. LDLNs were analyzed for spore+ cells at 6 hours post injection (h.p.i). Data from representative mice are plotted with gated frequencies. The y-axis represents an empty channel. (b) Calculated mean number of spore+ cells from each group in panel a. (c) C57BL/6 (n=3) or B6.CCR7−/− (n=4) mice were inoculated i.t. with a mixture of 1 × 108 BA spores and 1 × 108 FITC+ latex beads. LDLNs were analyzed by flow cytometry at 24 h.p.i. Spore+ (x-axis) and bead+ (y-axis) cells from representative mice are plotted with gated frequencies. Data in panels a–c are representative of two independent experiments. (d) Calculated mean number of spore+ (DyLight 649+) cells from each group in panel c. (e) Calculated mean number of Latex bead (FITC+) cells from each group in panel c. Data in panels d and e are pooled from two independent experiments with n=3–4 per group. **P<0.005; NS, not significant.
