Figure 3
Reduced expression of pathogen-recognition receptors on macrophages and neutrophils in allergic mice compared with phosphate-buffered saline (PBS) controls. Expression of surface Toll-like receptor (TLR)-2 involved in bacterial recognition on (a, b) airway neutrophils and (c, d) CD11c+ airway macrophages is shown as percentage of cells and histograms. Percentage and total cell count of (e, f) triggering receptor expressed on myeloid cells (TREM)1-positive neutrophils in the airways and (g, h) TREM2-positive alveolar macrophages (CD11c+) 78 h after final house-dust mite (HDM) exposure. (i) Expression of TLR-negative regulators in whole lung of allergic mice was compared with PBS controls. The level of different RNA transcripts, as indicated, was assessed by real-time PCR. All values were normalized against the expression of 18S and then to a control (PBS) sample. Percent of CD11c+ macrophages expressing (j) ST2L or (k) CD200R was determined by flow cytometry. Data presented as box and whisker plots with the median of n=5 mice in two independent experiments. *P<0.05 ; **P<0.01. S.pn, Streptococcus pneumoniae.
