Figure 4
Equivalent numbers of neutrophils recruited into the airways and lungs of control (phosphate-buffered saline (PBS)) and house-dust mite (HDM)–exposed mice after recombinant CXCL1 (KC) or macrophage inflammatory protein (MIP)-2 instillation. (a) An illustration of KC or MIP-2 with or without S. pneumonia (S.pn) administration protocol. (b) Airway and (c) lung neutrophil (Ly6G+) recruitment in HDM- or PBS-exposed mice were analyzed 6 and 24 h after intranasal (i.n.) administration of 1 μg of recombinant murine KC per mouse on day 0. Airway and lung bacterial titres were determined 6 h after (d) infection±KC or (e) MIP-2. (f, g) Administration of 1 μg recombinant murine KC (f) or MIP-2 (g) per mouse overcomes the inability of HDM-exposed mice to recruit neutrophils 6 h after bacterial infection. Box and whisker plots of five mice per group representative of two experiments. *P<0.05; **P<0.01. CFU, colony-forming units.
