Figure 4
Expression of TNF- α, Ifn-γ and major histocompatibility complex (MHC) class I and II complexes. (a) Jejunum, ileum, and colon gene expression levels of TNF- α and Ifn-γ, and MHC class I and II complex activators were analyzed in germfree and conventionalized mice at indicated days post conventionalization. Values are depicted as box and whisker diagrams (top-to-bottom, maximum value, upper quartile, median, lower quartile, and minimal value, respectively). Any data not included between the whiskers is plotted as an outlier with a dot. Significant differences between time points are indicated by distinctive characters above the measurement groups (P<0.05), (n=6–11/day). (b) Heat map generated from the significantly expressed MHC class I and II genes (P<0.05) between the germfree and conventionalized mice at the indicated time points (n=6–11/day).
