Figure 5
Regional variation of increasing T-cell numbers and maturation. Representative immunohistochemistry of (a) Cd8a, (b) Cd4 and (c) Cd3ɛ in ileal tissues from (A) germfree, (B) days 16 and (C) 30 post conventionalization, (n=4–6/day). Dot plots represent the expression levels of Cd8a, Cd4 and Cd3ɛ in jejunal, ileal, and colonic tissues in germfree and conventionalized mice at indicated days post conventionalization. Individual values and medians are shown. Significant differences between time points are indicated by distinctive characters above the measurement groups (P<0.05; n=6–11/day). All panels are shown at the same magnification; arrows indicate positively stained cells (brown color).
