Figure 2
Interleukin (IL)-17-producing lymphocytes upregulate genes essential for mucosal homeostasis. (a) The top 200 genes based on P value for the greatest contrast between IL17+ and IL17− lymphocytes from mesenteric lymph nodes of SIV-uninfected rhesus macaque (RM). The complete linkage method was used for hierarchical cluster analysis. Both rows and columns are clustered. Each row represents normalized expression value for a single gene, and each column represents a sample (data are based upon RNA extractions from IL17+ lymphocytes from two SIV-uninfected RM, left, and IL17− lymphocytes from three SIV-uninfected RM, right). (b) Fold change of selected genes that were upregulated by IL-17+ lymphocytes (top) or IL-17− lymphocytes (bottom). P values donated above each gene fold change. (c–d) The network (c) and pathway (d) from Ingenuity Pathway Analysis for selected genes is shown. Red indicates upregulation (fold change >=1.5); green indicates downregulation (fold change <=−1.5); grey indicates genes whose expression values are between −1.5 and 1.5. (c) Shape: triangle represents kinase; square represents cytokine; rectangle represents ligand-dependent nuclear receptor; diamond represents enzyme; trapezoid represents transporter; ellipse represents transcription regulator; circle represents others. HMG, hydroxy methyl glutaryl(-CoA synthase); BCG, bacille Calmette-Guerin (vaccine); IFN, interferon; TGF, transforming growth factor.
