Figure 6
CD103+ dendritic cells (DCs) directly promote IL17A and RORc expression by naïve CD4+ T cells. Naïve CD4+ T cells were cocultured under Th17 conditions with stimulatory anti-CD3 beads alone (control), with CD103+ DCs, or with CD103−CD11c+ DCs in the presence of interleukin (IL)-6 (a, c) or absence of IL-6 (b, d). Gene expression for IL17A (a, b) and RORc (c, d) was measured by ΔΔCt compared with controls. Cells were sorted from live, SIV− rhesus macaque (RM) mesenteric lymph nodes. Rh1 and Rh2 designate the two RMs used for this experiment.
