Figure 1
From: Cytosolic flagellin receptor NLRC4 protects mice against mucosal and systemic challenges
Flagellin-induced gene expression in colons of WT, TLR5-deficient (T5KO), NLRC4-deficient (N4KO), and TLR5/NLRC4-doubleKO (T5/N4-DKO) mice. Indicated strains of mice (n=3) were administered 10 μg of flagellin (FliC) in 0.2 ml PBS through intraperitoneal injection. One-hour later, mice were killed, colons immediately removed, and mRNA extracted and subjected to microarray analysis of global gene expression. (a) Transcription profiles in the colons of WT, T5KO, N4KO, and T5/N4-DKO mice have been compared with the colons from vehicle-treated genotype-matching mice. Heat map shows the results of all three mice per condition assayed. Illustration of genes induced with >1.5-fold change (in red) was built using the TreeView software (Berkeley, CA). (b–e) Quantitative reverse transcription-PCR (qRT-PCR) was used to confirm mRNA synthesis of selected genes. *P<0.05. PBS, phosphate-buffered saline; TLR5, Toll-like receptor-5; WT, wild type.
