Figure 3
E7-specific and total CD8 T cells in cervix-vagina (CV) of E7-immunized Trif knockout (KO), Cardif KO, and Trif–Cardif double-KO mice intravaginal (IVAG) challenged with CpG of PIC. Groups of Trif KO (left panels), Cardif KO (middle panels), and Trif–Cardif double-KO (right panels) mice were subcutaneously immunized with the adjuvanted E7 vaccine and 5 days later IVAG instilled with phosphate-buffered saline (PBS), PIC, or CpG. Mice were killed three days later, and cells recovered from individual CV were stained with CD8-APC antibodies and TetE749–57-PE or TetL1165-173-PE (as control). The percentages of TetE7+ CD8+ cells (a) and total CD8 T cells are indicated (b). The horizontal bars represent the mean percentages. Significant differences are indicated by *P<0.05, **P<0.01, and ***P<0.001.
