Figure 5
Expression of chemokine receptors and integrin/selectin ligands in the T cells from cervix-vagina (CV) upon intravaginal (IVAG) CpG. Groups of eight mice were subcutaneously immunized with the adjuvanted E7 vaccine, and 5 days later IVAG instilled with phosphate-buffered saline (PBS) (white symbols) or CpG (black symbols). Mice were killed 3 days later, at day 9, and flow cytometry staining and analysis were performed on cells recovered from pools of two CV. Dead cells were excluded with the aqua dead kit. The percentage of total CD4 (circle) or CD8 (triangle) T cells expressing the chemokine receptors CXCR3, CCR5, CCR2, and CCR7 (a) and integrin/selectin ligands: αL, α4, α4β7, L-selectin, PSGL-1, CD43, ESL, and PSL (b) among either total CD4 or CD8 T cells are shown. The horizontal bars represent the mean percentages. Data are representative of results obtained in two independent experiments. Significant differences between IVAG PBS and IVAG CpG treatments within each T-cell populations as well as between the CD4 and CD8 T cells upon IVAG PBS are indicated by *P<0.05, **P<0.01, and ***P<0.001.
