Figure 1
From: Gal-lectin-dependent contact activates the inflammasome by invasive Entamoeba histolytica
Entamoeba histolytica (Eh) in direct contact with macrophages induces the secretion of caspase-1-regulated cytokines. (a-c) PMA-differentiated THP-1 macrophages were stimulated with the indicated number of live ameba (Eh) or secreted components from live ameba for 3 h and cytokine secretion was measured by multiplex cytokine array. Live ameba selectively induced secretion of interleukin (IL)-1β (a), IL-1α (b), and fibroblast growth factor (FGF)-2 (c). (d) PMA-differentiated THP-1 macrophages were stimulated with increasing numbers of live ameba for 30 min. Secretion of processed caspase-1 (p35 subunit, p20/p22 subunit and N-terminal CARD domain) and active IL-1β (p17) were detected in cell supernatants (SN) by immunoblot. Total IL-1β (e) and IL-18 (f) secretion were measured by enzyme-linked immunosorbent assay (ELISA). (g) Lipopolysaccharide (LPS)-primed mouse bone marrow-derived macrophages (BDMs) were stimulated with increasing numbers of live ameba for 30 min. Secretion of processed caspase-1 (p10 subunit) and active IL-1β (p17) were detected in cell SNs by immunoblot. ***P<0.005 vs. negative control. n=4.
