Figure 2
From: Gal-lectin-dependent contact activates the inflammasome by invasive Entamoeba histolytica
Contact-dependent cytokine secretion induced by Entamoeba histolytica (Eh) is regulated by the inflammasome. PMA-differentiated THP-1 macrophages were pre-treated for 60 min with zVAD-fmk (100 μM) or YVAD-fMK (100 μM) and stimulated with 1.5 × 103 live ameba for 30 min. (a) Secretion of processed caspase-1 (p35 subunit, p20/p22 subunit and N-terminal CARD domain) and active interleukin (IL)-1β (p17) were detected in cell supernatants (SN) by immunoblot. Total IL-1β (b) and IL-18 (c) secretion were measured by ELISA. (d) Lipopolysaccharide (LPS)-primed Wt and caspase-1−/− bone marrow-derived macrophages (BDMs) were stimulated with 1.5 × 103 live ameba or nigericin (10 μM) for 30 min and secretion of processed caspase-1 (p10 subunit) and active IL-1β (p17) were detected in cell SNs by immunoblot. ***P<0.005 vs. negative control. n=4.
