Figure 3
From: Gal-lectin-dependent contact activates the inflammasome by invasive Entamoeba histolytica
Inflammasome activation by Entamoeba histolytica (Eh) requires intact ameba and direct contact. (a, b) PMA-differentiated THP-1 macrophages were exposed to 1.5 × 103 live ameba or equivalent amounts of concentrated secretory components, whole ameba lysate, ameba membrane fraction, or ameba cytosolic fraction for 30 min. (c, d) PMA-differentiated THP-1 macrophages were exposed to 1.5 × 103 live ameba for 30 min or the indicated number of live ameba placed on the top chamber of transwells for 2 h. (a, c) Secretion of processed caspase-1 (p35 subunit, p20/p22 subunit and N-terminal CARD domain) was detected in cell supernatants (SN) by immunoblot. (b, d) Total interleukin (IL)-1β secretion was measured by enzyme-linked immunosorbent assay (ELISA). ***P<0.005 vs. negative control. n=4.
