Figure 4
From: Gal-lectin-dependent contact activates the inflammasome by invasive Entamoeba histolytica
Direct Entamoeba histolytica (Eh) contact recruits active caspase-1 to the cell surface. (a, b) Confocal images of PMA-differentiated THP-1 macrophages showing caspase-1 activation after 30 min of exposure to live Eh or nigericin (10 μM) in which active caspase-1 were stained with an YVAD-florescent-labeled inhibitor of caspase (FLICA). Mammalian nuclei were labeled with 4,6-diamidino-2-phenylindole (DAPI; ameba nuclei are not stained by DAPI). Arrowhead points to contact between the macrophage and ameba surface. Note that active caspase-1 accumulates around the contact surface. Arrows point to macrophages that have been phagoyctosed by an ameba. (b) An ameba that ingested several macrophages. Scale bar=20 μm, Mφ, macrophage.
