Figure 5
From: Gal-lectin-dependent contact activates the inflammasome by invasive Entamoeba histolytica
Serum of patients with invasive amebiasis inhibits Entamoeba histolytica (Eh)-induced inflammasome activation. (a-d) Ameba were treated with pooled human immune sera of patients with amebic liver abscess or normal immune serum at 1:100 or the indicated dilution for 10 min at 4 °C before incubation with PMA-differentiated THP-1 macrophages for 30 min. (a) Secretion of processed caspase-1 (p35 subunit, p20/p22 subunit and N-terminal CARD domain) and (b) active interleukin (IL)-1β (p17) were detected in cell supernatants (SN) by immunoblot. Total IL-1β (c) and IL-18 (d) secretion were measured by enzyme-linked immunosorbent assay (ELISA). (e) Human immune serum was used to detect the 170 and 130 kDa forms of Eh Gal/GalNAc lectin (Gal-lectin; 1 μg per lane) or in the total lysates of Eh. ***P<0.005 vs. negative control. n=4.
