Figure 6
From: Gal-lectin-dependent contact activates the inflammasome by invasive Entamoeba histolytica
Surface-bound Entamoeba histolytica (Eh) Gal-lectin is required to activate the inflammasome. (a–d) Galactose (55 mM) was used to competitively inhibit Gal-lectin binding or glucose (55 mM) as an osmotic control was added to PMA-differentiated THP-1 macrophages or lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BDMs) and exposed to 1.5 × 103 Eh for 30 min. Secretion of processed caspase-1 (a, d) and interleukin (IL)-1β (d) was detected in cell supernatants (SN) by immunoblot. Secretion of IL-1β (b) and IL-18 (c) were measured by enzyme-linked immunosorbent assay (ELISA). ***P<0.005 vs. negative control. n=4.
