Figure 7
From: Gal-lectin-dependent contact activates the inflammasome by invasive Entamoeba histolytica
Surface-bound Entamoeba histolytica (Eh) Gal-lectin is required to activate the inflammasome. (a, b) Ameba were incubated with increasing concentrations of purified human colonic mucin for 10 min at 4 °C before incubation with PMA-differentiated THP-1 macrophages for 30 min. (c, d) Ameba were treated with polyclonal anti-Gal-lectin sera or normal rabbit sera at 1:100 with the indicated dilution for 10 min at 4 °C before incubation with PMA-differentiated THP-1 macrophages for 30 min. Secretion of processed caspase-1 (a, c) was detected in cell supernatants (SN) by immunoblot. Secretion of interleukin (IL)-1β (b, d) was measured by enzyme-linked immunosorbent assay (ELISA). ***P<0.005 vs. negative control. n=4. LPS, lipopolysaccharide.
