Figure 8
From: Gal-lectin-dependent contact activates the inflammasome by invasive Entamoeba histolytica
Adherence-mediating epitopes of Entamoeba histolytica (Eh) Gal-lectin regulate inflammasome activation. (a) Schematic diagram of the 170 kDa Gal-lectin extracellular domain and the regions recognized by the anti-Gal-lectin mAb 1G7, 3F4, 8C12, and H85. (b-e) Ameba were treated with monoclonal antibodies (mAbs) against the Gal-lectin at 1:100 dilution for 10 min at 4 °C before incubation with PMA-differentiated THP-1 macrophages or lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BDMs) for 30 min. (b, e) Secretion of processed caspase-1 and IL-1β was detected in cell supernatants (SN) by immunoblot. Secretion of IL-1β (c) and IL-18 (d) were measured by ELISA. ***P<0.005 vs. negative control. n=4.
