Figure 9
From: Gal-lectin-dependent contact activates the inflammasome by invasive Entamoeba histolytica
Adherence-mediating epitopes of Entamoeba histolytica (Eh) Gal-lectin regulate inflammasome activation. Amebas were treated with Gal-lectin inhibitory mAb 1G7 at 1:100 dilutions for 10 min at 4 °C before incubation with PMA-differentiated THP-1 macrophages where active caspase-1 is labeled by YVAD-FLICA. Mammalian nuclei are labeled with 4,6-diamidino-2-phenylindole (DAPI; ameba nuclei are not stained by DAPI). Confocal images showing Eh in contact (white arrow) with macrophages activated caspase-1 (center panel) whereas no Eh adhered to macrophages following treatment with mAb 1G7. The Eh+1G7 panel shows an Eh in close proximity with a macrophage (Mφ) in the absence of caspase-1 activation. Scale bar=20 μm.
