Figure 1
From: IL-10 modulates DSS-induced colitis through a macrophage–ROS–NO axis
Macrophage interleukin (IL)-10Rα expression protects mice from dextran sodium sulfate (DSS)-induced colitis. (a) IL-10RαDCdel, (b) IL-10RαBdel, (c) IL-10RαTdel, and (d) IL-10RαMdel mice or littermate Cre− (IL-10Rαfl/fl) controls (n=10/cohort) received 3% DSS solution in drinking water ad libitum for 5 days. Mean±s.e.m. percent of initial body weight is plotted. **P<0.01; † death event. (e) IL-10RαMdel and IL-10Rαfl/fl mice (n=10/cohort) were treated with 4% DSS for 5 days and survival monitored. (f) IL-10RαMdel mice and IL-10Rαfl/fl controls (n=10/cohort) were depleted of neutrophils with 1A8 antibody or received control rat immunoglobulin G (IgG) 1 day before 3% DSS administration. Data are representative of three independent experiments. (g) IL-10−/−, IL-10Rα−/−, and IL-10Rαfl/fl mice were treated with 3% DSS for 5 days and body weight monitored. Data are representative of three independent experiments. *P<0.05, **P<0.01 for IL-10RαMdel vs. IL-10Rαfl/fl. Significant differences between IL-10RαMdel, IL-10Rα−/−, and IL-10−/− cohorts are not seen.
